Ethylenediaminetetraacetic acid (EDTA) chinhu chinochera simbi. Chinonzi chelating zvinoreva kuti chinogona kusangana nemamwe maion esimbi uye chinoita kuti maion esimbi akasungwa asashande.

Tichishandisa hunhu uhwu hweEDTA, kune mhando mbiri dzekushandiswa mukuyedza;

Imwe mhando ndeye reverse application, iyo inodzivisa mamwe maitiro nekuvhiringidza kusungwa kwemaion esimbi.

Tora ioni: Mune mamwe maitiro, kusungwa kwema ioni esimbi kwakakosha. Tinobatanidza EDTA nema ioni aya esimbi kuti tidzivise maitiro aya.

Semuenzaniso, kuvharika kweropa kunoda kuti ma calcium ions abatanidzwe. Tinowedzera EDTA muropa kuti tiwedzere ma calcium ions neEDTA, nokudaro tichidzivirira kuvharika kweropa. Iyi ndiyo nheyo yeEDTA yekudzivirira kuvharika kweropa;

Semuenzaniso, kugadzirira mushonga wekubvisa calcium mukurapa kwezvirwere kunoshandisawo chelation effect yeEDTA. Tinoziva kuti kana nyama ine calcium yakawanda isina kubviswa calcium, zvichave zvakaoma kucheka kuita nyama yakazara pakucheka, saka tinofanira kubvisa calcium muganda tisati tacheka.

Kunewo rudzi rwekushandiswa kwemberi kunokurudzira mamwe maitiro nekuvhiringidza kusungwa kwemaion esimbi.

Semuenzaniso, wedzera EDTA mumushonga wekugaya trypsin. Kana EDTA isina kuwedzerwa, trypsin inosunga calcium nemagnesium pamusoro pemasero kana munzvimbo yekurera, nokudaro ichidzivisa kushanda kwetrypsin.

Panguva ino, wedzera EDTA shoma kuti EDTA ikwanise kusangana necalcium, magnesium nedzimwe ions dze divalent. Panguva ino, panowanikwa mamiriro akanaka ekuti trypsin iwanikwe.

Chinhu chinoshamisa zvikuru nezveEDTA ndiko kunyungudika kwayo. Inonyungudika zvishoma mumvura uye inoita sechena mumvura. Panguva ino, wedzera sodium hydroxide kuti ugadzirise pH yayo. Sezvo pH ichiwedzera, mhinduro yacho inova yakajeka zvikuru. Kana pH yava pedyo ne8, inova yakajeka chose. Haityi acid asi alkali.

EDTA ine mashandisirwo akawanda akanaka. Zvinogona kuonekwa kuti zvinhu zviri muzvisikwa zvine mashiripiti, asi hatina kuzviona.

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