Ethylenediaminetetraacetic acid (EDTA) isimbi chelating agent. Iyo inonzi chelating inoreva kuti inogona kusanganisa nemamwe maion esimbi uye kuita kuti iyo yakasungwa simbi ions isashande.

Uchishandisa hunhu uhu hweEDTA, kune marudzi maviri ezvikumbiro mukuedza;

Imwe mhando ndeye reverse application, iyo inodzivisa kumwe kuita nekukanganisa kusungwa kwemaoni esimbi.

Tora ion: Mune mamwe maitiro, kusungirirwa kwesimbi ions kwakakosha. Isu tinobatanidza EDTA neaya simbi ions kuvharidzira kuita uku.

Semuenzaniso, kubatanidza ropa kunoda kubatanidzwa kwe calcium ions. Isu tinowedzera EDTA kuropa kuti chelate calcium ions neEDTA, nokudaro kudzivirira ropa coagulation. Iyi ndiyo nheyo yeEDTA anticoagulation;

Kune mumwe muenzaniso, kugadzirira kwe decalcification solution mu pathological staining inoshandisawo chelation effect yeEDTA. Isu tinoziva kuti kana yakakwira-calcium tishu isina decalcified, zvichave zvakaoma kucheka kuita matishu akazara kana slicing, saka isu tinoda decalcify tisati slicing.

Kune zvakare mhando yekumberi application inosimudzira mamwe maitiro nekukanganisa kusungwa kwesimbi ions.

Semuenzaniso, wedzera EDTA kune trypsin digestion solution. Kana EDTA ikasawedzerwa, trypsin inosunga kucalcium uye magnesium pachisero kana mutsika yepakati, nekudaro ichivharira trypsin kuita.

Panguva ino, wedzera zvishoma zveEDTA kuti ubvumire EDTA kuti ibatanidze neaya emahara calcium, magnesium uye mamwe divalent ions. Panguva ino, mamiriro akanaka anogadzirwa kuitira kuitika kwetrypsin.

Chinhu chinonyanya kushamisa nezve EDTA ndiko kunyungudika kwayo. Inonyungudika zvishoma mumvura uye inoita seichena mumvura. Panguva ino, wedzera sodium hydroxide kugadzirisa yayo pH kukosha. Sezvo iyo pH inowedzera uye yakakwirira, mhinduro inova Inova yakajeka uye yakajeka. Kana iyo pH iri pedyo ne8, inova yakanyatsojeka. Haisi kutya asidi asi alkali.

EDTA ine zvakawanda zvinoshamisa zvekushandisa. Zvinogona kuonekwa kuti zvinhu zvakasikwa zvine mashiripiti, asi isu hatina kuzviona.

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