1. Trihydroxymethyl aminomethane yekutungamira kugadzirwa kwemaindasitiri

Nekuda kwechimiro chayo chiri nyore, atomu yekabhoni iri pakati pechinhu ine chimiro che tetrahedral, ine matatu hydroxymethyl uye boka reamino, rinogona kuita zvakasiyana-siyana zvekutsiva uye REDOX reactions, imhando yezvinhu zvemhando yepamusoro zvekugadzira makemikari. Imwe yenzira dzekugadzira buffer TAPS yakagadzirwa uchishandisa Tris uye 1, 3-propanolone mualcohol. Kazhinji, zvinhu zveTRIS zvinoshandiswa pakugadzira maindasitiri hazvidi kuchena kwakanyanya kwe reagent. Kusimbiswa kuri pahuwandu hwakawanda, saka zvinodhura zvishoma kuwana mugadziri wezvinhu zvekugadzira ane simba guru.

2. Trimethylol amino methane yekutarisa nzira yekuongorora biochemical

Tris inowanzo shandiswa semushonga wezvinhu zvipenyu kuchengetedza pH yemaitiro emakemikari. Nekuda kwekusasimba kwealkalinity, Tris inowanzo shandiswa pamwe chete nemaacids. Inonyanya kushandiswa pa nucleic acid, protein dissolving buffer, protein imprinting buffer, DNA imprinting experiment buffer, DNA agarose gel electrophoresis buffer, nezvimwewo. Mu reaction system nekuda kwekubatanidzwa kwema enzymes, mapuroteni nema antibodies akasiyana-siyana, saka zvinodiwa zvemushonga zvakakwirira, saka kutenga kunofanirwa kusarudza mugadziri ane makore mazhinji ekutsvagisa nekugadzira uye ruzivo rwekugadzira, kuchena kwakanyanya, kusvibiswa kushoma kuti ave nechokwadi chekururama kwekuyedza kwemakemikari. Tris buffer tembiricha emhedzisiro, nyore kunyudza carbon dioxide mumhepo, dhizaini yemushonga inofanira kuvharwa zvakasimba.

3. Trimethylol aminomethane yekutsvagisa nekugadzira mishonga

Matatu ehydroxymethyl aminomethane ane zita rekuti trometamol, sezvo isina sodium amino buffer base, mumuviri inopindura neH2CO3, inoderedza H2CO3, inogadzira HCO32 - zvakare panguva imwe chete, inogona kupinza mahydrogen ions uye kugadzirisa acidemia, simba rayo rakasimba, uye inogona kuburikidza nemembrane yesero, inowanzoshandiswa mu acute metabolic ne respiratory acidemia; Semushonga wealkaline, unoshandiswa kugadzirisa acidosis pasina kukonzera kuwedzera kwekuchengetedza carbon dioxide. Nekuti inoshandiswa pamishonga, kugadzirwa kwese kweTRIS raw material source, nzira yekugadzira, kuchena kwechigadzirwa, kusvibiswa kwehuwandu hweion zvinodiwa zvakanyanya, kutenga rudzi urwu rwezvigadzirwa kunofanirwa kuve kwakasimba uye kungwarira, kunofanirwa kupasa bvunzo dzakasimba.

4. Trimethylaminomethane inoshandiswa mumvura inochengeterwa mavhairasi

Mukugadzirisa hutachiona, Tris inogona kutanga yachengetedza pH yemuenzaniso, ichishanda se biological buffer, uye pH buffer range yeTris ndeiyi: 7.0 kusvika 9.0, kugona kuchengetedza kubvisa kuputika kwemuenzaniso mushure mekuburitswa kwekugadzikana kwe nucleic acid, kudzivirira kuora kwe nucleic acid, uye kuvandudza huwandu uye kuchena kwe nucleic acid, kuvimbisa kururama kwemabasa anotevera akadai se nucleic acid testing analysis, nekuda kwenguva yakakosha yedenda, zvinodiwa zve nucleic acid detection, huwandu hwekutenga kweTris huchava hukuru, saka tinokurudzira kutenga zvigadzirwa zvakadaro kutanga tatenga huwandu hushoma hwechigadzirwa usati watenga kuyedzwa kukuru kwakasimbiswa kuti hapana dambudziko, zvakare inofanira kuva nehunyanzvi hwekutengesa mushure mekutengesa kuti ipe rutsigiro rwehunyanzvi rwehunyanzvi.